FIGURE 3.

Apc inactivation correlates with constitutive CpG methylation of Fxr in adjacent colonic mucosa and colon tumors of Apc^Min/+ mice. (A) Bars represent qRT-PCR quantitation (fold of C57BL/6J) using methylation-specific primers of Fxr-M/β-actin in NT colonic mucosa and colon tumors from Apc^Min/+ mice. Values are means ± SEMs, n = 4 (mean of 6 replicates/mouse). Means without a common letter differ, P < 0.05. (B) Position of CpGs in exon-3 of the mouse Fxr gene. Black circles indicate methylated and white circles indicate unmethylated CpGs of 4 clones with 10 replicates per clone from 4 independent colon tumors. Apc, adenomatous polyposis coli; Fxr, farnesoid X receptor; Fxr-M, Fxrα3/4 promoter methylation.