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. 2019 Jul 10;13:316. doi: 10.3389/fncel.2019.00316

FIGURE 1.

FIGURE 1

Intercellular mitochondrial transfer between astrocytes. (A) Schematic showing that human astrocytes (HA) were labeled with GFP or mitodsred and then co-cultured at a ratio of 1:1. (B) Time-lapse live images showing mitochondria from HA-mitodsred dynamically transferring into HA-GFP. (C) Confocal images showing dsred-labeled mitochondria in HA-GFP after co-cultured for 24 h. (D,E) Immunostaining of TOM20 (D) and ATP5A1 (E) on transferred mitochondria in HA after co-cultured for 24 h. (F) Flow cytometry analyzing mitochondrial transfer efficiency between HA after co-cultured for 24 h. (G) Astrocytes (Ast) were derived from H1 ESCs with NFIB and SOX9 and then labeled with GFP or mitodsred lentivirus. (H) Confocal images showing that mitochondria from Ast-mitodsred were observed in Ast-GFP after co-cultured for 24 h. (I) Flow cytometry analyzing mitochondrial transfer efficiency between Ast after co-cultured for 24 h.