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. 2019 Jul 10;13:316. doi: 10.3389/fncel.2019.00316

FIGURE 2.

FIGURE 2

Mitochondrial transfer from neuronal cells into astrocytes. (A) Schematic showing that HA-GFP were co-cultured with SK-mitodsred at a ratio of 1:1. (B) Time-lapse live images showing mitochondria from SK-mitodsred dynamically transferring into HA-GFP. (C) Confocal images showing mitochondria from SK-mitodsred in HA-GFP after co-cultured for 24 h. (D–F) Immunostaining of TOM20 (D), ATP5A1 (E), and LAMP1 (F) after co-cultured for 24 h. (G) Flow cytometry analyzing mitochondrial transfer efficiency from SK into HA after co-cultured for 24 h. (H) H1 ESC-derived astrocytes (Ast) or neurons (hN) were labeled with GFAP::GFP or SYN1::mitoBFP. (I) Confocal images showing that mitochondria from hN-mitoBFP were observed in Ast-GFP after co-cultured for 48 h. (J) Flow cytometry analyzing mitochondrial transfer efficiency from hN cells into Ast after co-cultured for 48 h.