Figure 5.

Expression of TLR7 in splenocytes is modulated by disease and Tg7 in a leukocyte specific manner. Splenocytes from 6 to 7months old B6, Sle1, Sle1Tg7, Sle1IRAK4^KD/KD, and Sle1Tg7IRAK4^KD/KD female mice were analyzed for intracellular TLR7 expression using flow cytometry. TLR7^−/− mice and fluorescent minus one (FMO) controls were used to determine specificity. (A) Cumulative data of TLR7 expression in B cells, (B) plasmablasts, (C) plasma cells, and (D) CD115⁺Gr1⁻ monocytes (Gr1⁻ Monos), (E) F4/80⁺CD11b^lo macrophages (Macs), (F) B220⁺SiglecH⁺mPDCA1+ pDCs, (G) CD115⁺Gr1⁺ monocytes (Gr1⁺ Monos), (H) CD11b⁺CD11c⁺MHCII⁺ DCs (CD11b⁺DCs), across all strains. (I) Correlation of TLR7 expression in B cells, CD11b⁺ cDCs, pDCs and macrophages from Sle1Tg7 mice with effector memory CD4⁺ T frequencies in the spleen. Data from 6 to 10 mice per group is shown. Parametric data were assessed by 1-way ANOVA (with Bonferroni's multiple comparisons test) and non-parametric data with Kruskal-Wallis (with Dunn's multiple comparisons test); ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. Additional comparisons between two groups were made with a Student's t test or Mann-Whitney test for parametric and non-parametric data, respectively, indicated by # (^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001). Correlations were performed by calculating Pearson's correlation coefficients (r) and their significance assessed by a two-tailed t-test.