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. 2019 Jul 9;12:2019–2030. doi: 10.2147/IDR.S215084

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© 2019 Swedan et al.

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Representative gel electrophoresis result for WLBU2 DNA binding assay. DNA binding assay was utilized to determine the ability of WLBU2 to bind bacterial plasmid DNA (pUC19 plasmid, 2686 base pairs); L: 1 KB DNA ladder (values are in base pairs); C: DNA alone (8.33 ng/µL); from 1 to 8 twofold diluted WLBU2 (200–1.563 µM or 680–5.313 µg/mL) combined with fixed DNA concentration (8.33 ng/µL); Electrophoresis was done at 140 V for 45 mins on 1% agarose; DNA was visualized under a UV transilluminator; no band shifts occurred for lanes 1–8 indicating no binding between WLBU2 and DNA.