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. 2019 Jul 8;12:1985–2001. doi: 10.2147/IDR.S209881

Table 1.

Primers used for sil genes detection with the corresponding annealing temperatures and the expected amplicon size

Gene Forward primer Reverse primer Annealing temperature (°C) Amplicon size (bp)
silE aggggaaacggtctgacttc atatccatgagcgggtcaac 52 221
silB caaagaacagcgcgtgatta gctcagacattgctggcata 52 233
silP cctgggtttacagcgtcatt atggcacctgaggtttgttc 52 175
silF cgatatgaatgctgccagtg attgccctgctgaataaacg 52 229
silA cttgagcatgccaacaagaa ctgccagtacaggaaccat 54 162
silRS gacggcaatcgcaatcagatt gtggaggatactgcgagagc 54 192
silCBA cgggaaacgctgaaaaattatgaa gtacgttcccagcaccagtt 54 189