Figure 4.

In vitro and in vivo efficacy of drug-loaded liposomes in RCC cell lines. 786-O (A) and A498 (B) cells were treated with various drug-loaded TTP-conjugated Liposomes for 72 hrs. Then, cell viability was determined with MTS assay. Dual drug-loaded liposomes showed higher reduction in cell viability compared to single drug-loaded liposomes in all cell lines. (C) 5×10⁶ 786-O cells were subcutaneously injected into the right flanks of 8 weeks old male SCID mice. Tumors were allowed to grow until the average tumor size is ~400–500 mm³. Then, mice were treated with drug-loaded liposomes (one mouse per treatment group) 3x/wk for 3 weeks. Tumors were measured weekly and tumor volume is plotted to obtain the respective growth curves. In both cases, dual-drug-loaded liposomes demonstrated significant inhibition compared to single drug-loaded liposomes. Some of the mice were sacrificed before the completion of experiment due to ulceration of tumors. (D) Similar results were obtained in A498 xenografts.