Figure 6.

Validation of the result obtained from single mouse trial in cohorts of 5 mice. (A) 5×10⁶ 786-O cells were subcutaneously injected into the right flanks of 8 weeks old male SCID mice. Tumors were allowed to grow until the average tumor size is ~300 mm³. Then, mice were treated with vehicle or EV-L (five mice per treatment group) 3x/wk for 3 weeks. Tumors were measured weekly and tumor volume is plotted to obtain the respective growth curves. EV-L demonstrated significant inhibition compared to the vehicle group. (B) Images of the harvested tumors at the end of the experiment. (C) Representative images of H&E and Ki67 staining of the tumor tissue sections. Bar length =200 µm. (D) Quantification of Ki67 positive nuclei. *** denotes p<0.001 compared to control.

Abbreviation: H & E, hematoxylin and eosin.