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. 2019 Jul 1;2019:2490761. doi: 10.1155/2019/2490761

Figure 1.

Figure 1

c-Jun is a direct target of miR-214. (a) Schematic illustration of the hypothetical duplexes formed through interactions between the binding site in the c-Jun 3′-UTR and miR-214. (b) RT-qPCR analysis of miR-214 levels in hAMSCs and SCLCs. (c) RT-qPCR analysis of miR-214 levels in SCLCs that were transfected with nc, miR-214, or anti-miR-214. (d, e) Western blotting analysis of c-Jun protein levels in SCLCs that were transfected with nc, miR-214, or anti-miR-214. GAPDH was used as the internal control: (d) representative image; (e) quantitative analysis. (f) Relative luciferase signals from SCLCs that were cotransfected with luciferase reporter gene (wt) or mutated luciferase reporter gene (mut) and nc, miR-214, or anti-miR-214. Data are shown as mean ± SEM (n = 3). P < 0.05, relative to nc.