Figure 2.

Validation of SCLCs that are derived from hAMSCs through differentiation. (a, b) Western blotting analysis of S100β, GFAP, and p75 protein levels in hAMSCs after differentiation in SC induction medium for 0, 7, 13, and 19 days and in SCs. GAPDH was used as the internal control: (a) representative image; (b) quantitative analysis. (c) Immunofluorescence staining of S100β, GFAP, and p75 in undifferentiated hAMSCs or SCLCs that were hAMSCs after 19 days' differentiation. Scale bar: 50 μm. (d) Concentration of BDNF and GDNF in the culture supernatants of hAMSCs after differentiation in SC induction medium for 0, 7, 13, and 19 days and in the culture supernatants of SCs. Data are shown as mean ± SEM (n = 3).