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. 2019 Jul 10;10:901. doi: 10.3389/fpls.2019.00901

Figure 4.

Figure 4

Effects of crude CLP extracts co-applied with M. oryzae spores on rice leaves on rice blast disease. (A) Effects of co-application of N3, WLIP, lokisin, entolysin, xantholysin, and orfamide extracts with fungal spores on rice blast caused by M. oryzae. Disease assessment was done at 6 days post-inoculation by counting the number of sporulating susceptible-type lesions occurring on the second youngest leaf of the rice plants. Data of one experiment made up of three biological replicates, consisting of 12 plants each are shown. The experiment was repeated with comparable results. Different letters indicate statistically significant differences among treatments (ANOVA followed by a Tukey’s tests: n = 36; α = 0.05). (B) Representative disease symptoms on second youngest leaf after 6 days post-inoculation with M. oryzae. Scale bar represents 1 cm. DC, disease control; HC, healthy control; Lok, lokisin; Ent, entolysin; Xan, xantholysin; Orf, orfamide; BTH, S-methyl 1,2,3-benzothiadiazole-7-carbothioate.