Table 5.
Comparison of the oligopeptide sequence and fatty acid moiety of CLPs used in this study.
| CLPs | FAa | 1 | 2 | 3b | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | L:rc | References |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WLIP | 3-OH C10 | L-Leu | D-Glu | D-aThr | D-Val | D-Leu | D-Ser | L-Leu | D-Ser | L-Ile | 9:7 | This study | |||||
| Orfamide A | 3-OH C14 | L-Leu | D-Glu | D-aThr | D-Ile | L-Leu | D-Ser | L-Leu | L-Leu | D-Ser | L-Val | 10:8 | Ma et al., 2016a | ||||
| Lokisin | 3-OH C10 | D-Leu | D-Asp | D-aThr | D-Leu | D-Leu | D-Ser | L-Leu | D-Ser | L-Leu | L-Ile | L-Asp | 11:9 | This study | |||
| Entolysin A | 3-OH C10 | Leu | Glu | Gln | Val | Leu | Gln | Val | Leu | Gln | Ser | Val | Leu | Ser | Ile | 14:5 | Oni et al., 2019a |
| Xantholysin A | 3-OH C10 | Leu | Glu | Gln | Val | Leu | Gln | Ser | Val | Leu | Gln | Leu | Leu | Gln | Ile | 14:8 | Oni et al., 2019a |
The amino acid configuration of orfamide A is based on the absolute configuration elucidated for strain Pf-5. The amino acid stereochemistry of lokisin (COR10) is predicted from C-domain phylogenetic analysis (Supplementary Figure S3 and Table 4). aFA, fatty acid. baThr, allo-threonine. cL, length of peptide in each CLP; r, number of amino acids involved in the lactone ring of each CLP (residues underlined).