Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 9;12:5485–5497. doi: 10.2147/OTT.S197009

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Ma et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

DANCR directly targeted miR-34a-5p. (A) The putative binding sites of DANCR in miR-34a-5p were predicted using DIANA-LncBase software. (B and C) Luciferase activity of wild-type or mutant DANCR in DU145/DTX and PC3/DTX cells following miR-NC or miR-34a-5p transfection was determined by luciferase reporter assay. (D) The correlation between DANCR and miR-34a-5p in DU145/DTX and PC3/DTX cells was detected by RNA immunoprecipitation (RIP) assay. (E and F) The direct interaction between DANCR and miR-34a-5p was assessed by RNA pull-down assay. (G) The miR-34a-5p level in DTX-sensitive or DTX-resistant PC cells was detected by qRT-PCR. (H and I) The expression of DANCR and miR-34a-5p in DU145/DTX and PC3/DTX cells, which were initially transfected with pcDNA, pcDNA-DANCR, si-NC, or si-DANCR, was determined by qRT-PCR assay. *p<0.05.

Abbreviations: DANCR, anti-differentiation noncoding RNA; DTX, docetaxel; MUT, mutant; PC, prostate cancer; qRT-PCR, quantitative reverse transcription PCR; si-DANCR, small interfering RNA targeting DANCR; WT, wild type.