Table 1.
Systems | Number of test cases |
Reported map resolution range (Å) |
Mean reported map resolution (Å) |
Local map resolution range (Å) |
Mean local map resolution (Å) |
Mean of the best RMSD of top 10 scoring models (Å) |
Mean convergence estimate (Å) |
---|---|---|---|---|---|---|---|
Small RNPs, lower-resolution simulated maps1 | 20 | 5.0–7.0 | 6.0 | 5.0–7.0 | 6.0 | 2.4 | 3.5 |
Small RNPs, higher-resolution simulated maps1 | 10 | 3.0 | 3.0 | 3.0 | 3.0 | 1.4 | 2.5 |
Large RNPs, lower-resolution experimental maps2 | 19 | 4.5–10.5 | 8.4 | 5.0–12.4 | 9.5 | 4.9 | 7.5 |
Large RNPs, higher-resolution experimental maps3 | 6 | 2.9–3.7 | 3.5 | 2.9–5.7 | 4.1 | 2.4 | 3.4 |
Blind tests, experimental maps4 | 6 | 5.4–6.0 | 5.9 | 6.6–7.3 | 6.7 | 3.6 | 5.9 |
Small RNPs: E. coli L25–5S rRNA (1dfu), Sex-lethal RRM (1b7f), Ribotoxin restrictocin – SRL analog (1jbs), SmpB-tmRNA complex (1p6v), HutP antitermination complex (1wpu), mRNA binding domain of SelB elongation factor (1wsu), NusA transcriptional regulator (2asb), Methyltransferase RumA in complex with rRNA (2bh2), PP7 coat protein and viral RNA (2qux), Puf4 bound to 3’ UTR of target transcript (3bx2). Complete data is provided in Supplementary Table 1 and Supplementary Table 3.
Large RNPs built into lower-resolution experimental maps: U4/U6.U5 tri-snRNP U4/U6 3WJ, U5 3WJ, U5 IL II; mitochondrial ribosome loop 1, loop 2; CRISPR-Cas9; MS2 packaged genome S1+S2, S3, S4, S5+S6, S7, S8, S9–1, S9–2, S10, S12, S15+S16; Yeast U1 snRNP yeast-specific 4WJ, SL2–2. Complete data is provided in Supplementary Table 1.
Large RNPs built into higher-resolution experimental maps: U4/U6.U5 tri-snRNP U4/U6 3WJ, U5 3WJ, U5 IL II; mitochondrial ribosome loop 1, loop 2; CRISPR-Cas9. Complete data is provided in Supplementary Table 3.
Blind tests: Yeast U1 snRNP core 4WJ, core 4WJ only, yeast 3WJ, yeast-specific 4WJ, SL2–2; Yeast P complex ligated exon. Complete data is provided in Supplementary Table 1.