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. 2019 Jul 5;15(7):e1008224. doi: 10.1371/journal.pgen.1008224

Fig 6. Mutation of Asp429 to Lys increases taurocholate-independent germination.

Fig 6

(A) Space fill model of CspC highlighting the residues flanking the short loop containing R456 and G457 (orange spheres) juxtaposed to D429 and Q516 (blue spheres). A shallow cavity on the surface of the subtilase-like domain is visible between these four residues. The image is rendered to enhance cavity contrast using PyMOL (Schrödinger, Inc.) (B) Germinant sensitivity of cspC mutant spores encoding mutations in Asp429 and Gln516 when plated on BHIS containing increasing concentrations of taurocholate. The number of colony forming units (CFUs) that arose from germinating spores is shown in two graphs to improve readability. The mean and standard deviations shown are based on three replicates performed on three independent spore purifications. Lower error bars have been omitted to improve readability. Statistical significance relative to wild type was determined using a two-way ANOVA and Tukey’s test. **** p < 0.0001, * p < 0.05. (B) Western blot analyses of CspC and CspB levels in D429 and Q516 mutant spores. CotA serves as a loading control. The results are representative of three biological replicates performed on three independent spore preps.