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. 2019 May 16;39(7):1379–1389. doi: 10.1161/ATVBAHA.118.312272

Figure 2.

Figure 2.

Atherosclerotic B cell transgenic (Btg) mice display reduced IgG antibody responses. Antibody-producing B cell subsets and serum antibody levels were quantified (by flow cytometry and ELISA, respectively) in tissues from western diet–fed Apoe−/− or Btg Apoe−/− mice. A, Bone marrow (BM) plasma cells (B220− CD138hi). Data from 1 Apoe−/− mouse were excluded during data collection. B and C, Total serum IgG and IgM levels. D, Spleen germinal center (GC) B cell levels (CXCR5+ GL7+). E, Spleen B-1 cell levels (CD19+ B220+/lo IgM+ CD43+ CD23−). F and G, Total serum IgG2c and IgG1. Data from 1 Apoe−/− mouse were excluded during data collection. H, Serum MDA-LDL-binding IgG2c or IgG1. Data from 1 Apoe−/− mouse were excluded during data collection. I, IgM antibodies to MDA-BSA, PC-BSA, or the T15 idiotype (AB1-2 antibodies). *P<0.05, **P<0.01, ***P<0.005. BSA indicates bovine serum albumin; LDL, low-density lipoprotein; MDA, malondialdehyde; PC, phosphorylcholine RLU, relative light units; and WT, wild-type.