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Fluctuation analysis of fluorescent variants of mORC4 in embryos.
| Injected RNAa | Ave. Diffusion (μm2 s−1)b | Diffusion 1 (μm2 s−1) | Diffusion 2 (μm2 s−1) | Concentration (nM)b |
|---|---|---|---|---|
| EGFP | 9.5 ± 1.0 | N/A | N/A | 120 ± 20 |
| mORC4-EGFP | 5.0 ± 0.5 | 7.5 ± 1.0 | 0.8 ± 0.3 | 40 ± 10 |
| mORC4-FlAsH | 6.0 ± 1.0 | 7.0 ± 0.8 | 0.6 ± 0.2 | 160 ± 50 |
For each condition, n = 10.
Values were calculated using SimFCS to fit correlation functions.
