Table 2.
Phasor point distribution of fluorescent variants of mORC4 in embryos at different activation stages.
| Injected protein(s)a | Phasor angleb | Major axis (a)b | Minor axis (b)b | Ratio (a/b) |
|---|---|---|---|---|
| ORC4-EGFP (AII) | 75 ± 5 | 0.47 ± 0.07 | 0.20 ± 0.02 | 2.3 ± 0.3 |
| ORC4-EGFP (G1) | 49 ± 4 | 1.00 ± 0.10 | 0.18 ± 0.03 | 5.6 ± 1.0c |
| ORC4-FlAsH (AII) | 18 ± 5 | 0.43 ± 0.10 | 0.17 ± 0.05 | 2.5 ± 0.4 |
| ORC4-FlAsH (G1) | 36 ± 8 | 0.73 ± 0.10 | 0.10 ± 0.04 | 7.3 ± 2.0c |
a
For each condition, n = 10.
b
Calculated using SimFCS as described previously [27].
c
p < 0.05 from a student t test where identical mRNA injections were compared between activation states.