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. Author manuscript; available in PMC: 2019 Jul 17.
Published in final edited form as: Cell Rep. 2018 Feb 13;22(7):1835–1848. doi: 10.1016/j.celrep.2018.01.052

Figure 5. Inhibition of JNK Prevents Inflammasome Activation in PTPN2-LysMCre Mice.

Figure 5.

(A) BMDMs from WT, PTPN2-LysMCre, and ASC–/– mice were left untreated or activated with MSU for 2 hr and lysates analyzed for JNK and Syk phosphorylation and expression of PTPN2, ASC, and NLRP3. Each lane represents an individual mouse.

(B and C) BMDMs from WT and PTPN2-LysMCre (KO) mice were treated with Syk, JNK, or p38 inhibitors 1 hr prior to activation with MSU. Analysis of the cell culture supernatant for IL-1β secretion (B) and maturation (C) by ELISA and western blot, respectively.

(D) Precipitation of ASC from the cell lysate and analysis for tyrosine phosphorylation and co-precipitated NLRP3 and PTPN2.

Asterisks denote significant differences (*p < 0.05 and **p < 0.01; Student’s t test with Bonferroni correction). Error bars represent means ± SD. See also Figure S7.

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