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. Author manuscript; available in PMC: 2019 Jul 17.
Published in final edited form as: Cell Rep. 2018 Feb 13;22(7):1835–1848. doi: 10.1016/j.celrep.2018.01.052

Figure 7. Inhibition of IL-1α Protects WT Mice from Tumor Induction.

Figure 7.

(A) Serum from WT and PTPN92-LysMCre mice with chronic DSS colitis was analyzed for IL-1α levels. Dashed line, detection limit of the ELISA.

(B and C) Peritoneal macrophages and intestinal epithelial cells (lECs) from WT and PTPN2-LysMCre mice were analyzed for IL1a surface expression (B) and calpain mRNA levels (C).

(D–F) WT and PTPN2-LysMCre mice were immunized with IL-1α-coupled Qβ virus-like particles (IL-1α VLP) or with control Qβ virus-like particles 5,3, and 1 week prior to start of colitis and/or tumor induction. (D) Weight development of mice in acute colitis, (E) representative pictures of H&E-stained slides, and

(F) quantification of histology score of terminal colon pieces from WT and PTPN2-LysMCre mice subjected to chronic colitis.

(G-I) Representative pictures of colonoscopy (G), observed tumor number in dissected colon (H), and average tumor size (I) in each mouse in the AOM/DSS tumor model.

Results are representative of one of two independent experiments with three to five mice per group. Asterisks denote statistical significance (*p < 0.05; MannWhitney U test with Bonferroni correction). Scale bars represent 100 mm. Error bars represent means ± SD.

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