FIG. 3.

Activation of RANKL in HEK293T cells with multiplex TREE system. (A) Schematic illustration of the positions of sgRNAs used for the activation of RANKL. Protospacer and PAM sequences are shown as blue and yellow boxes, respectively. See also Supplementary Figure S4A. (B) Schematic illustration of luciferase reporter vector containing RANKL promoter and 5stratio (C) Activity comparisons among the effectors and the repeat numbers of GCN4 epitopes, as well as the MS2-effector system, by the reporter assay. Data are shown as the mean ± SD (n = 4). **p < 0.01. (D) Endogenous RANKL expression quantified by qPCR. Data are shown as the mean ± SD (n = 4). **p < 0.01.