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. 2019 Jul 1;8:e46315. doi: 10.7554/eLife.46315

Figure 4. Elevated Hipk and perturbations in cell signaling cascades induce dMyc accumulation in the wing hinge region.

(a) dMyc staining (gray) in a control wing disc. (b) Schematic diagram of a larval wing imaginal disc. The hinge region can be divided into dorsal, lateral and ventral compartments. (c–g) dMyc staining (gray) in flip-out clones expressing hipk (c), yki-S168A (d), Arm-S10 (e), Ci (f) or N-act (g) under the control of actin-Gal4. RFP (red) marks the transgene-expressing clones. DAPI staining for DNA (blue) reveals tissue morphology. (c’, c’’-g’, g’’) are magnified images of the insets (dashed lines) in (c-g). (c) White arrowhead indicates dMyc accumulation in a hipk-expressing clone. (d) White arrowheads indicate yki-S168A-expressing clones with dMyc accumulation. (e) White arrowheads indicate ectopic dMyc expression at the boundary of the Arm-S10-expressing clone and the neighboring wild-type cells. (f) White arrowheads indicate dMyc upregulation in Ci-expressing clones. (g) White arrowheads indicate dMyc accumulation in N-act-expressing clones and the adjacent wild-type cells. Scale bars, 50 μm.

Figure 4.

Figure 4—figure supplement 1. Effects on dMyc expression by elevated Hipk or perturbations in cell signaling.

Figure 4—figure supplement 1.

(a–h) dMyc staining (gray) in flip-out clones expressing hipk (a–d), yki-S168A (e), Arm-S10 (f), Ci (g) or N-act (h) under the control of actin-Gal4. RFP (red) marks the transgene-expressing clones. DAPI staining for DNA (blue) reveals tissue morphology. (a’, a’’-h’, h’’) are magnified images of the insets (dashed lines) in (a–h). Scale bars, 50 μm.