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. 2019 Jul 17;5(7):eaav8152. doi: 10.1126/sciadv.aav8152

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Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

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Fig. 2 — (A) Data for the ear thickness of WT or Cd19^Cre mice with CHS for 4 days after challenge with OXZ are shown. (B) The histograms show the numbers of IL-13⁺ ILC2s isolated from the spleen, aLN, iLN, and ear tissue 2 days after the OXZ challenge. (C) Data for the ear thickness and (D) the number of IL-13⁺ ILC2s in Cd19^Cre mice with CHS are shown after the adoptive transfer of OXZ-sensitized WT or Il10^−/− B cell subsets as indicated. (A to D) The results are expressed as the mean ± SEM from two independent experiments (n = 5 per group for each experiment). *P < 0.05; **P < 0.01; n.s., not significant by Student’s t test (A to C) or one-way analysis of variance (ANOVA) with post hoc Tukey’s test (D). (E to G) The histograms for (E) the number of tissue IL-13⁺ ILC2s, (F) the ear thickness, and (G) the histology images of ear tissues (2 days after OXZ challenge; scale bars, 200 μm) in CHS Rag2^−/− mice with or without anti-Thy1.2 mAb treatment are shown. The results are expressed as the mean ± SEM from two independent experiments (n = 4 per group for each experiment). *P < 0.05; **P < 0.01; n.s., not significant versus Rag2^−/− + isotype OXZ by Student’s t test. (H) After the adoptive transfer of WT splenic CD19⁺ B cells into Rag2^−/− mice, CHS was induced by OXZ challenge. Representative immunofluorescence images of IL-10 (green) and B220 (red) are shown (n = 5; scale bars, 100 μm). (I) Ear thicknesses and (J) IL-13⁺ ILC2 numbers in CHS Rag2^−/− mice with or without the transfer of WT or Il10^−/− CD1d^hiCD5⁺ B cell subsets. (K) Representative flow cytometry images for IL-13⁺ ILC2s in coculture with WT CD1d^hiCD5⁺, WT non-CD1d^hiCD5⁺, or Il10^−/− CD1d^hiCD5⁺ B cell subsets at a 1:1 ratio and (L) frequency data are shown. (I to L) The results are expressed as the mean ± SEM (I, J, and L) or representative images (K) from two independent experiments (n ≥ 3 per group for each experiment). *P < 0.05; **P < 0.01; n.s., not significant by Student’s t test (I) or one-way ANOVA with post hoc Tukey’s test (J and L).