Fig. 4. Reconstitution of MCs in Kit^W-sh/W-sh mice increases IL-10⁺ B_reg cells and suppresses the CHS response.

(A) The histograms show the frequencies of total CD19⁺ B cells in peripheral tissues from WT mice or Kit^W-sh/W-sh mice with or without the adoptive transfer of MCs. (B and C) Representative plot images show IL-10⁺ CD19⁺ B cells (B), and histograms show the frequencies of CD5⁺CD19⁺ B cells (C) in peripheral tissues from WT mice or Kit^W-sh/W-sh mice with or without the adoptive transfer of MCs. (D) Ear thickness, (E) the frequency of IL-10⁺ B cells, and (F) the number of IL-13⁺ ILC2s are shown during CHS in WT or Kit^W-sh/W-sh mice with or without the transfer of MCs. Data are expressed as the mean ± SEM (A and C to F) and representative images (B) from two independent experiments (n ≥ 3 per group for each experiment). *P < 0.05; **P < 0.01; n.s., not significant by Student’s t test (D and F) or one-way ANOVA with post hoc Tukey’s test (C and E). BMMC, bone marrow-derived MC; PDMC, PeC-derived MC.