Figure 4. Temporal fluctuations of RBC flux and Mean-PO₂ within individual capillaries in cortical layers I-V.

Panels (a–b) and (c–d) show the layer-dependent standard deviations (STDs) and coefficients of variance (CVs) of the temporal fluctuations of RBC flux and Mean-PO₂, respectively. The STD and CV of each observable for each capillary were calculated based on the 9-s-long time course. The analysis in (a–d) was made with 130, 140, 63 and 40 samples, collected in cortical layers I, II/III, IV and V, respectively, across n = 7 mice. Each sample corresponds to a 9-s-long, 15-time-point measurement acquired in each capillary. Data are expressed as mean ± SEM. Statistical comparisons were carried out using ANOVA followed by Tukey HSD post hoc test. The asterisk symbol (*) indicates p<0.05.

Figure 4—figure supplement 1. Temporal fluctuations of RBC speed within individual capillaries in cortical layers I-V.

Panels (a–b) show the layer-dependent standard deviations (STDs) and coefficients of variance (CVs) of the temporal fluctuations of RBC speed. The STD and CV of each observable for each capillary were calculated based on the 9-s-long time course. This analysis was made with 130, 140, 63 and 40 samples, collected in cortical layers I, II/III, IV and V, respectively, across n = 7 mice. Each sample corresponds to a 9-s-long, 15-time-point measurement acquired in each capillary. Data are expressed as mean ± SEM. Statistical comparisons were carried out using ANOVA followed by Tukey HSD post hoc test. The single-asterisk symbol (*) indicates p<0.05.