Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Protein (Homo sapiens) | PKM2 | N/A | Uniprot ID: P14618-1 | |
| Cell line (Homo sapiens) | HCT116 | ATCC, Manassas, VA, USA | ATCC Cat# CCL-247, RRID:CVCL_0291 | |
| Cell line (Homo sapiens) | LN229 | ATCC, Manassas, VA, USA | ATCC Cat# CRL-2611, RRID:CVCL_0393 | |
| Cell line (Homo sapiens) | SN12C | Kaelin Lab, DFCI, Boston, MA, USA | RRID:CVCL_1705 | |
| Antibody | Rabbit Anti-PKM2, Clone D78A4 | Cell Signaling Technology | Cat# 4053; RRID:AB_1904096 | 1:1000 in 5% BSA/T-BST |
| Recombinant DNA reagent | pET28a-His-PKM2(WT) | Anastasiou et al., 2011 | RRID:Addgene_42515 | Available from AddGene (Cambridge MA, USA). See Supplementary file 2 for PKM2 sequence. |
| Recombinant DNA reagent | pET28a-His-PKM2(I124G) | This study | 1-step mutagenesis using pET28a-His-PKM2(WT) as template | |
| Recombinant DNA reagent | pET28a-His-PKM2(F244V) | This study | 1-step mutagenesis using pET28a-His-PKM2(WT) as template | |
| Recombinant DNA reagent | pET28a-His-PKM2(R489L) | This study | 1-step mutagenesis using pET28a-His-PKM2(WT) as template | |
| Recombinant DNA reagent | pET28a-His-PKM2(K305Q) | This study | 1-step mutagenesis using pET28a-His-PKM2(WT) as template | |
| Recombinant DNA reagent | pET28a-His-PKM2(F307P) | This study | 1-step mutagenesis using pET28a-His-PKM2(WT) as template | |
| Recombinant DNA reagent | pET28a-His-PKM2(A327S) | This study | 1-step mutagenesis using pET28a-His-PKM2(WT) as template | |
| Recombinant DNA reagent | pET28a-His-PKM2(C358A) | This study | 1-step mutagenesis using pET28a-His-PKM2(WT) as template | |
| Peptide | ITLDNAYMEK[13C615N2] | This study | Synthesised by the Crick Peptide Synthesis STP | |
| Peptide | GDLGIEIPAEK[13C615N2] | This study | Synthesised by the Crick Peptide Synthesis STP | |
| Peptide | APIIAVTR[13C615N4] | This study | Synthesised by the Crick Peptide Synthesis STP | |
| Peptide | LFEELVR[13C615N4] | This study | Synthesised by the Crick Peptide Synthesis STP | |
| Peptide | LAPITSDPTEATAVGAVEASFK[13C615N2] | This study | Synthesised by the Crick Peptide Synthesis STP | |
| Chemical compound | Potassium phosphoenolpyruvate (2,3-13C2) | Cambridge Isotope Laboratories | CLM-3398-PK | |
| Chemical compound | D-fructose 1,6-bisphosphate sodium salt hydrate (U-13C6) | Cambridge Isotope Laboratories | CLM-8962-PK | |
| Chemical compound | L-Phe (ring-13C6) | Cambridge Isotope Laboratories | CLM-1055-PK | |
| Chemical compound | L-Ser (13C6) | Cambridge Isotope Laboratories | CLM-1574-H | |
| Software | sa_encode.R | This study | Software to encode trajectory into stacked alignment of structural alphabet strings (Kleinjung, 2019; copy archived at https://github.com/elifesciences-publications/ALLOHUBMAT) | |
| Software | kabsch.R | This study | Kabsch superpositioning routine (Kleinjung, 2019; copy archived at https://github.com/elifesciences-publications/ALLOHUBMAT) | |
| Software | MI.R | This study | Mutual Information and other entropy metrics between two character vectors, here intended for two alignment columns (Kleinjung, 2019; copy archived at https://github.com/elifesciences-publications/ALLOHUBMAT) | |
| Software | Xcalibur QualBrowser | Thermo Fisher Scientific (Waltham MA, USA) | N/A | |
| Software | Tracefinder v4.1 | Thermo Fisher Scientific (Waltham MA, USA) | N/A |