Table 1. Apparent steady-state binding constants of PKM2 for FBP, Phe and Ser.
Titrated ligand | Constant ligand | KDapparent |
---|---|---|
FBP | – | (21.4 ± 9.0) nM |
Ser (5 mM) | (12.5 ± 7.7) nM | |
Phe (1.5 mM) | (8.1 ± 5.6) nM | |
Phe | – | (191.0 ± 86.3) µM |
FBP (50 μM) | (132.0 ± 11.7) µM | |
Ser | – | (507.5 ± 218.2) µM |
FBP (50 μM) | (462.0 ± 97.1) µM |