Table 2. Intracellular concentrations of FBP, Phe and Ser in cancer cell lines.
| Metabolite | Media condition | Cell line | [Metabolite]ic (µM) | Fractional saturation |
|---|---|---|---|---|
| FBP | Gluc-
(RPMI) |
HCT116 | 38.4 ± 16.6 | 0.99 ± 0.00 |
| LN229 | 73.4 ± 18.4 | 0.99 ± 0.00 | ||
| SN12C | 19.9 ± 7.5 | 0.98 ± 0.00 | ||
| Gluc+
(RPMI) |
HCT116 | 238.4 ± 67.4 | 0.99 ± 0.00 | |
| LN229 | 302.0 ± 75.3 | 0.99 ± 0.00 | ||
| SN12C | 355.1 ± 66.2 | 0.99 ± 0.00 | ||
| Gluc+ aa-
(HBSS) |
HCT116 | 337.2 ± 185.5 | 0.99 ± 0.00 | |
| LN229 | 257.0 ± 33.9 | 0.99 ± 0.00 | ||
| SN12C | 236.8 ± 41.1 | 0.99 ± 0.00 | ||
| Gluc+ Phe500 Ser100
(HBSS) |
HCT116 | 384.5 ± 41.8 | 0.99 ± 0.00 | |
| LN229 | 277.3 ± 83.4 | 0.99 ± 0.00 | ||
| SN12C | 300.3 ± 89.1 | 0.99 ± 0.00 | ||
| Gluc+ Phe100 Ser500
(HBSS) |
HCT116 | 400.9 ± 82.2 | 0.99 ± 0.00 | |
| LN229 | 372.2 ± 106.0 | 0.99 ± 0.00 | ||
| SN12C | 286.4 ± 73.6 | 0.99 ± 0.00 | ||
| Phe | Gluc-
(RPMI) |
HCT116 | 256.3 ± 65.8 | 0.56 ± 0.06 |
| LN229 | 231.6 ± 22.6 | 0.55 ± 0.03 | ||
| SN12C | 100.4 ± 24.6 | 0.34 ± 0.06 | ||
| Gluc+
(RPMI) |
HCT116 | 258.2 ± 79.6 | 0.56 ± 0.08 | |
| LN229 | 318.7 ± 115.8 | 0.61 ± 0.09 | ||
| SN12C | 197.5 ± 26.6 | 0.51 ± 0.04 | ||
| Gluc+ aa-
(HBSS) |
HCT116 | 41.1 ± 15.8 | 0.17 ± 0.05 | |
| LN229 | 31.7 ± 28.0 | 0.13 ± 0.12 | ||
| SN12C | 10.6 ± 0.6 | 0.05 ± 0.02 | ||
| Gluc+ Phe500 Ser100
(HBSS) |
HCT116 | 1776.2 ± 225.1 | 0.90 ± 0.01 | |
| LN229 | 1666.5 ± 543.0 | 0.89 ± 0.04 | ||
| SN12C | 926.1 ± 252.7 | 0.82 ± 0.04 | ||
| Gluc+ Phe100 Ser500
(HBSS) |
HCT116 | 582.4 ± 23.8 | 0.75 ± 0.01 | |
| LN229 | 575.7 ± 224.4 | 0.73 ± 0.11 | ||
| SN12C | 221.6 ± 49.1 | 0.53 ± 0.06 | ||
| Ser | Gluc-
(RPMI) |
HCT116 | 3580.9 ± 1016.9 | 0.87 ± 0.03 |
| LN229 | 1755.0 ± 159.5 | 0.77 ± 0.02 | ||
| SN12C | 854.3 ± 240.7 | 0.62 ± 0.07 | ||
| Gluc+
(RPMI) |
HCT116 | 3943.5 ± 1363.1 | 0.88 ± 0.05 | |
| LN229 | 2226.7 ± 757.7 | 0.80 ± 0.06 | ||
| SN12C | 1766.0 ± 142.9 | 0.78 ± 0.01 | ||
| Gluc+ aa-
(HBSS) |
HCT116 | 426.9 ± 179.2 | 0.44 ± 0.09 | |
| LN229 | 249.6 ± 221.8 | 0.28 ± 0.25 | ||
| SN12C | 111.0 ± 18.4 | 0.18 ± 0.02 | ||
| Gluc+ Phe500 Ser100
(HBSS) |
HCT116 | 6157.3 ± 1334.4 | 0.92 ± 0.01 | |
| LN229 | 2641.1 ± 825.1 | 0.83 ± 0.06 | ||
| SN12C | 2197.7 ± 605.8 | 0.81 ± 0.04 | ||
| Gluc+ Phe100 Ser500
(HBSS) |
HCT116 | 22360.4 ± 1554.2 | 0.98 ± 0.00 | |
| LN229 | 8464.7 ± 3214.5 | 0.93 ± 0.04 | ||
| SN12C | 6603.9 ± 1500.4 | 0.93 ± 0.02 |