. 2019 Jul 2;8:e45068. doi: 10.7554/eLife.45068

Table 3. Steady-state Michaelis-Menten kinetic parameters for PKM2^apo, and following the addition of FBP, Phe and Ser.

PKM2^ligand	K_M^PEP (mM)	k_cat (s⁻¹)	k_cat/K_M^PEP (s⁻¹ mM⁻¹)
PKM2^apo	1.22 ± 0.02	349.3 ± 40.9	285.6 ± 34.1
PKM2^Phe	7.08 ± 1.58	324.7 ± 23.9	46.8 ± 6.0
PKM2^Ser	0.22 ± 0.04	323.1 ± 43.2	1489.8 ± 84.7
PKM2^FBP	0.23 ± 0.04	356.7 ± 25.7	1540.4 ± 96.9
PKM2 ^{FBP + Phe}	0.65 ± 0.03	222.3 ± 6.3	342.1 ± 11.1
PKM2 ^{FBP + Ser}	0.20 ± 0.04	348.7 ± 44.3	1620.0 ± 253.6

Table 3. Steady-state Michaelis-Menten kinetic parameters for PKM2apo, and following the addition of FBP, Phe and Ser.