Figure 2.

R17 decreases lipid synthesis and cellular TG accumulation induced by FAs or glucose. Panel (a), effects of R17 in HF‐fed mice as described for Figure 1. (a) Effects on the expression of key lipogenic proteins in the liver. Animal experiments were described in Section 2 and Figure 1 legend. The extracted protein from the liver samples were immunoblotted with indicated specific antibodies and quantified to the loading control of GAPDH. Data shown are individual values with means ± SEM, N = 8 mice per group. *P < .05, significantly different from CH control mice; ^# P < .05, significantly different from HF control mice. Panels (b–e), effects of R17 in cultured HuH7 cells. (b–c) Effects of R17 (0.4, 1 μM) on TG level and expression of lipogenic proteins in cultured HuH7 cells incubated with oleic acid (OA, 0.5 mM) or palmitic acid (PA, 0.5 mM) for 24 hr. Protein levels were quantified and normalized to actin; and relative fold increases were determined by comparison with the blank group. (d–e) Effect of R17 on the TG level and expression of lipogenic proteins in high glucose stimulated HuH7 cells. Cells cultured at 25‐mM glucose was primed at 5‐mM glucose for 8 hr followed by the stimulation of lipogenesis with 30‐mM glucose for 24 hr. The blank control group was cultured at 25‐mM glucose throughout the experiment. The extracted protein from the cells were immunoblotted with indicated specific antibodies. Protein levels were quantified and normalized to actin; and relative fold increases were determined by comparison with the blank group. Data shown are individual values with means ± SEM, N = 5 independent experiments. *P < .05, significantly different from blank control; ^# P < .05, significantly different from the corresponding group of OA, PA, or 30‐mM glucose in the absence of R17