Figure 7.

Inhibition of PPARδ/DHFR attenuated the effects of GW501516 on NO and ROS production. BAECs were pretreated with GSK0660 (2 μmol·L⁻¹) or MTX (1 μmol·L⁻¹) for 1 hr, before coincubation with GW501516 (1 μmol·L⁻¹) for 12 hr, and then exposed to PA (200 μmol·L⁻¹) for 12 hr. (a) Representative images of DAF‐FM DA fluorescence signal in ECs in response to A23187 (1 μmol·L⁻¹) under a confocal microscope, analysed by comparing fluorescence intensity before (F0) and after (F1) the addition of A23187. Summarized results showing the levels of NO production in BAECs starting from the addition of A23187 for 210 s. Scale bar: 50 μm (n = 5). (b) NO production was measured with EPR using Fe(DETC)₂. (c) Confocal microscopic detection of superoxide with DHE (n = 5). (d) Production of ROS was measured with L‐012 chemiluminescence (n = 5). (e) HPLC analysis of 2‐hydroxyethidium levels (n = 5). *P < .05 versus vehicle; ^# P < .05 versus PA; ^† P < .05 versus GW501516 + PA