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. 2019 Jul 17;9:10329. doi: 10.1038/s41598-019-46949-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cellular localization of p97. (A) MCF-10A cells were plated for 24 hours and then treated with buffer or the indicated concentrations of CB-5083 (p97i). 24 hours after treatment, cells were visualized by immunofluorescence, with representative images shown (A). (B) Quantitation of relative nuclear abundance of p97 in (A), normalized to the mean in buffer-treated cells. (C,D) MCF-7 cells were treated, visualized (C), and quantitated (D), as in (A,B). Doses above 1.0 μM CB-5083 show elevated levels of the ER-associated stress and apoptosis marker CHOP (Fig. S4C). For each condition, three biological replicates were performed and a total of ≥40 cells were counted. Box plots are drawn from the first to third quartile and the median value is marked by a horizontal line. Whiskers extend to the minimum and maximum values. *p < 0.05, **p < 0.005, ***p < 0.0005, not significant (ns) p > 0.05.