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. 2019 Jul 17;9:10329. doi: 10.1038/s41598-019-46949-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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p97-R155C is functionally defective for chromatin extraction. (A) pDNA was incubated in extract supplemented with p97-WT, p97-D1D2, or p97-R155C. DNA-bound proteins were isolated by plasmid pull-down at various times and visualized by Western blot with the indicated antibodies. Note that p97 antibodies recognize both endogenous and recombinant proteins. (B,C) Relative quantitation of total DNA-bound p97 (B) and ubiquitin (C) from three biological replicates. Values are normalized to peak accumulation in the +p97-WT reaction and error bars represent +/− one standard deviation. Full Western blot images are available in Fig. S7.