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. 2019 Jul 17;9:10326. doi: 10.1038/s41598-019-46876-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative methylation levels of mSEPT9 in colorectal cancer at different time points. Relative methylation of mSEPT9 (line = median) in colorectal cancer (CRC) patients (a) with or without recurrence; (b) with or without new metastases; (c) with or without death; (d) with poor or better prognosis during follow-up after operation. Poor prognosis refers to the presence of any of the three mentioned prognostic factors, while better prognosis refers to none of them. The P value was calculated by paired t test through GraphPad Prism (version 7; GraphPad Software, San Diego, CA) and represented the significance level of the difference in each group as aforesaid. The dots represent the mean value of SEPT9 methylation level in three PCR reactions for each individual patient, while the dotted lines show the trend of the median methylation levels with time. Pre = pre-operation, M = months.