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. 2019 Jul 17;9:10349. doi: 10.1038/s41598-019-46699-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SRC-3 expression pattern and AKT/mTOR signaling pathway component expression in normal and PE human placentas. (A) IF staining of SRC-3 (green) and CK7 (red) in frozen sections of human term-placentas; nuclei were counterstained by DAPI (blue). Scale bars: 200 μm. (B) Western blots of SRC-3 in human term-placentas, n = 5, *p < 0.05. (C) Western blots of AKT, p-AKT, mTOR, and p-mTOR protein expression in human term-placentas. β-actin served as a loading control, n = 6, *p < 0.05. All experiments were repeated at least three times.