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. 2019 Jul 17;9:10383. doi: 10.1038/s41598-019-46875-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of quality metric across library preparation methods. (a) Raw reads with sequencing quality > Q20 or >Q30. (b) Bases trimmed due to low quality (<Q20). (c) Library insert size (bp). (d) Bases containing adaptor sequences. (e) Overlapping bases between Read 1 and Read 2. (f) Read duplicates (read-pairs). Read duplicates were defined as two read-pairs with the same start and end positions. All libraries were sequenced on the HiSeq X platform. n.s.: not statistically significant, P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001. Bars show average values, with error bars representing standard error of mean.