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. 2019 Jul 17;9:10373. doi: 10.1038/s41598-019-46793-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stratification and differentiation of the LSC sheets in the presence of DAPT and SAHM1. (a) Representative images of double K14-K12 staining using different concentrations of DAPT/SAHM1 before and after air-lifting (AL) induction. (b) Quantification of the number of layers (stratification) in the presence of DAPT/SAHM1 before and after AL. Data are represented as mean ± SEM. Abbreviations: AL, air-lifting; K12, cytokeratin 12; K14, cytokeratin 14; LSC, limbal stem/progenitor cells.