Skip to main content
. 2019 Jul 17;5:117. doi: 10.1038/s41420-019-0199-x

Fig. 3. Mitochondrial fission mediated by A-1210477 and S63845 occurs in a DRP-1-dependent manner.

Fig. 3

a H1299 cells were exposed to Z-VAD.fmk (30 µM) for 0.5 h, followed by either A-1331852 (100 nM), A-1210477 (10 µM), S63845 (100 nM), or a combination of the different inhibitors for 4 h and assessed for mitochondrial integrity by immunostaining with HSP70 antibody. b H1299 cells were transfected with control or DRP-1 siRNA for 72 h and exposed to A-1210477 (10 µM) or S63845 (1 and 10 µM) for 4 h and assessed for mitochondrial integrity. The extent of mitochondrial fragmentation was quantified by analysing ~100 cells for each condition in three independent experiments. Scale bar: 10 µm. Error bars = mean ± SEM. Statistical analysis was conducted by one-way ANOVA (***P ≤ 0.001)