Fig. 6. DRP-1 regulates BH3 mimetic-induced activation of BAK.
a Scheme demonstrating the primary mechanism of action of BH3 mimetics and the downstream events that culminate in apoptosis. b Immunoprecipitation of BCL-XL and MCL-1 was carried out in H1299 cells, transfected with control or DRP-1 siRNA, followed by exposure to Z-VAD.fmk (30 µM) for 0.5 h and a combination of A-1331852 (100 nM) and A-1210477 (10 µM) for 4 h, and the eluted complexes were immunoblotted for the indicated proteins. c H1299 cells were treated as b and the extent of BAK activation was assessed by flow cytometry from at least three independent experiments. Error bars = mean ± SEM. Statistical analysis was conducted by one-way ANOVA (***P ≤ 0.001). d Western blots of different molecular weight fractions from size exclusion chromatography showing BAK oligomerisation in H1299 cells upon exposure to A-1331852 (100 nM) and A-1210477 (10 µM) for 2 h. e Immunoprecipitation of active BAK in H1299 cells treated as b, and the eluted complexes were immunoblotted for the indicated proteins. f Representative images of cells showing BAK activation and DRP-1 distribution in H1299 cells treated as b. Scale bar: 10 µm