Table 2.
Mean and SEM of sperm quality attributes (sperm membranes integrity and function, production of ROS, lipid peroxidation and DNA fragmentation) evaluated in the selected commercial semen batches.
| Sperm quality analyses of the selected batches | Fertility groups | |
|---|---|---|
| HFa (n = 3) | LFb (n = 3) | |
| Sperm membranes integrity, mitochondrial membrane function and ROS production by fluorescence microscopy | ||
| PIAIHc (%) | 48.9 ± 4.7 | 45.2 ± 6.1 |
| Plasma membrane integrity (%) | 51.5 ± 5.1 | 48.5 ± 6.4 |
| Acrosome membrane integrity (%) | 60.7 ± 3.6 | 59.4 ± 4.1 |
| High mitochondrial membrane potential (%) | 62.6 ± 7.9 | 54.7 ± 7.2 |
| ROSd (%) | 5.2 ± 0.9 | 10.7 ± 4.9 |
| Sperm membranes integrity, mitochondrial membrane function, ROS production, lipid peroxidation and DNA fragmentation by flow cytometry | ||
| Plasma membrane integrity (%) | 49.5 ± 0.3 | 45.6 ± 8.2 |
| Acrosome membrane integrity (%) | 83.9 ± 2.3 | 78.3 ± 3.7 |
| Plasma and acrosome membrane integrity (%) | 49.3 ± 0.3 | 45.4 ± 8.3 |
| Plasma membrane integrity and high mitochondrial membrane potential (%) | 24.4 ± 2.1 | 26.3 ± 2.9 |
| Plasma membrane integrity and mitochondrial positive ROS (%) | 5.3 ± 1.6 | 4.1 ± 1.0 |
| Mitochondrial positive ROS (ua) | 621.0 ± 76.2 | 678.3 ± 23.5 |
| Lipid peroxidation (ua) | 2,502.0 ± 395.6 | 2,764.9 ± 921.8 |
| DNA fragmentation (%) | 5.8 ± 0.1 | 5.7 ± 0.1 |
Differences were considered significant when P < 0.05. aHigh fertility; bLow fertility; cSperm with plasma and acrosome membrane integrity and high mitochondrial potential; dProduction of ROS (reactive oxygen species). SEM: standard error of the mean.