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. 2019 Jul 17;9:10350. doi: 10.1038/s41598-019-46601-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Timely AKI to CKD transition detection by serpinA3. (A) SerpinA3 mRNA levels in renal cortex during the follow-up of the animals, (B) SerpinA3 protein levels in the renal cortex evaluated by Western Blot 4 months post-ischemia, (C,D) representative microphotographs of serpinA3 immunostaining in renal cortex from the control group, magnification 400 and 800x respectively, (E) Urinary serpinA3 levels during AKI to CKD transition and compared with the control group, (F) Spearman correlation between urine serpinA3 and tubulo-interstitial fibrosis, and (G,H) representative microphotographs of serpinA3 immunostaining in renal cortex from the AKI to CKD transition group, magnification 400 and 800x respectively. Data are represented as mean ± SE. (for sham, n = 4, and for the AKI to CKD transition groups, n = 5 per period). White circles or bar represent sham and black circles or bar represent AKI to CKD transition groups and. *p < 0.05 vs. the sham group in their respective period, ^ϕp < 0.05 vs. IR + UNx 2^nd month and ωp < 0.05 vs. IR + UNx 3^rd month.