Fig. 6.

Point mutations in D2 and D3 lead to loss of function. SKOV3 cells were transduced with an empty vector (CTRL), wild-type (OPCML) or mutant (R71C, E201Q, S203R, R214Q, K230R, K239N, M278I) OPCML and analyzed for protein expression by western blot (a) and protein expression/localization by confocal microscopy (b) with an anti-OPCML antibody. GAPDH was used as loading control in a. Scale bar = 5 μm. Cells were then tested for migration (c) and invasion (d) in transwell assays and for anchorage-independent growth in agarose (e). Scale bar = 50 μm (c, d) and 200 μm (e). All the graphs show the mean ± s.e.m. of three independent experiments. Student t-test compares CTRL and mutants to wild-type OPCML: **p < 0.01, ***p < 0.001, ****p < 0.0001