Fig. 7.

Different domains mediate distinct functions. a SKOV3 cells transduced with an empty vector (CTRL), wild-type (OPCML) or mutant (R65L, N70H, R71C, P95R, P95L, P95S in D1 in the left panel, E201Q, S203R, R214Q in D2 in the central panel, K230R, K239N, M278I in D3 in the right panel) OPCML were analyzed for proliferation. CTRL cells are represented in magenta, wild-type OPCML in black and the mutants in light blue. All the graphs show the mean ± s.d. of four independent experiments. b–e The different cell lines were plated onto collagen I (b), collagen IV (c), fibronectin (d) or laminin (e), and adhesion was tested after 1 h. Values have been normalized to CTRL. Scale bar = 50 μm. All the graphs show the mean ± s.e.m. of three independent experiments for domains 1 and 2 independent experiments for domains 2 and 3. Student t-test compares CTRL and mutants to wild-type OPCML: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001