Fig. 8.

Point mutations impair OPCML tumor suppressor function in in vivo models. a SKOV3 cells expressing empty vector (CTRL), wild-type (OPCML) or mutant (R65L, N70H and P95R) OPCML were injected intraperitoneally in five female athymic mice each. Tumors and ascites were collected and measured in grams and milliliters, respectively, as indicated. The mean is indicated by a line in the dot plots. Student t-test compares CTRL and mutants to wild-type OPCML: *p < 0.05, **p < 0.01. Scale bar = 1 cm. b SKOV3 cells expressing GFP and the indicated constructs were grown embedded in Matrigel on top of chick chorio–allantoic membranes. Tumors were excised, imaged and the GFP-positive area measured. Scale bar = 500 μm. The graph shows the mean ± s.e.m. of three eggs. Student t-test compares CTRL and mutants to wild-type OPCML: ***p < 0.001, ****p < 0.0001