Figure 1.

Downregulation of Mitochondrial Cytb Was Linked to Increased ROS in db/db Mouse Heart

(A) ROS was detected by DHE probe in frozen heart sections of db/db mice in comparison with wild-type controls. n = 3. (B–D) Western blot was performed to analyze the nuclear DNA encoded ETC subunits (B), mitochondrial DNA encoded ETC subunits (C), and statistical analysis (D) in the heart of db/db mice. (E) Quantitative real-time PCR was used to determine the mRNA levels of ETC subunits in the heart of db/db mice. (F) Mitochondrial ROS levels were detected by MitoSOX red in H9c2 cells transfected with si-ND1, si-Cytb, si-COI, or si-ATP6. n = 3; results from controls were set to 1; *p < 0.05 versus si-NC.