Figure 4.

Overexpression of miR-92a-2-5p but Not let-7b-5p Alleviated Cardiac Dysfunction in db/db Mice

(A) Overview of the experimental setup for rAAV-miR-92a-2-5p and rAAV-let-7b-5p treatment (n = 8). (B) Mitochondrial miR-92a-2-5p and let-7b-5p levels were determined by quantitative real-time PCR in various groups. (C) Cytb protein in the heart of db/db mice were detected by western blot. (D–I) Comparison of echocardiographic and hemodynamic measurements including E/A ratio (D), dp/dt (E), heart rate (F), EF value (G), LVIDd (H), and LVIDs (I) in rAAV-miR-92a-2-5p- and rAAV-let-7b-5p-treated db/db mice were shown. (J and K) Body weight (J) and blood glucose (K) in various groups were analyzed every 4 weeks. (L and M) H&E and WGA (L) and oil red and DHE staining (M) of myocardial tissue were detected in db/db mice. *p < 0.05 versus wild-type, ^#p < 0.05 versus db/db-rAAV-random.