Figure 6.

The influence of UDCAs on the FXR signalling pathway in vitro and CCK fold change in vivo. (a) Representative pictures of protein expression of the FXR signalling pathway components and the bile acid transporter ASBT from six samples each group in 3D cultured intestine cells. (b) Immunofluorescent staining of FXR signalling pathway components in 3D cultured intestine cells and mean fluorescence intensity shown as a bar chart (FGF19 n = 15 per group, FXR n = 6 per group). (c) Fold change in CCK postprandial versus fasting condition (n = 5 per group). (UDCAs means the mixture of TUDCA, GUDCA, UDCA, TLCA, and LCA at a concentration of 50, 50, 12.5, 2.5, and 0.25 μM; DCAs means the mixture of TCA, TDCA, GDCA, and DCA at a concentration of 50, 12.5, 12.5, and 0.5 μM.) Data are mean values ± SEM. *P < .05 versus normal control group