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. 2016 Apr 9;33(4):281–286. doi: 10.5511/plantbiotechnology.16.0218a

Figure 1. Quantitative analysis of the localization patterns of ARA6-GFP/ARA6-mRFP and ARA6-RFP/GFP-PTS1 in root epidermal cells near meristematic regions of A. thaliana. (A–C) Comparison between subcellular localization of ARA6-GFP (A, green) and ARA6-mRFP (B, magenta). (C) Merged image of (A) and (B). Scale bar=5 µm. (D–F) Subcellular distribution of peroxisomes (GFP-PTS1) (D, green) and ARA6-mRFP (E), magenta. (F) Merged image of (D) and (E). Scale bar=5 µm. (G) Stack bar graph representing the relationships of ARA6-GFP/ARA6-mRFP and ARA6-mRFP/GFP-PTS1. The distances from the center of each ARA6-GFP spot to the center of the closest ARA6-mRFP spot (left bar) and the distances from the center of each ARA6-mRFP spot to the center of the closest ARA6-GFP spot (middle bar) were classified as described previously (Ito et al. 2012). The graph shows that there were no differences between the two measurements (p>0.5 by Student’s t-test). On the other hand, the frequency of colocalization was significantly lower for ARA6-mRFP/GFP-PTS1 (right bar) (p***<0.001 by Tukey’s test). Error bars indicate±standard deviation.

Figure 1. Quantitative analysis of the localization patterns of ARA6-GFP/ARA6-mRFP and ARA6-RFP/GFP-PTS1 in root epidermal cells near meristematic regions of A. thaliana. (A–C) Comparison between subcellular localization of ARA6-GFP (A, green) and ARA6-mRFP (B, magenta). (C) Merged image of (A) and (B). Scale bar=5 µm. (D–F) Subcellular distribution of peroxisomes (GFP-PTS1) (D, green) and ARA6-mRFP (E), magenta. (F) Merged image of (D) and (E). Scale bar=5 µm. (G) Stack bar graph representing the relationships of ARA6-GFP/ARA6-mRFP and ARA6-mRFP/GFP-PTS1. The distances from the center of each ARA6-GFP spot to the center of the closest ARA6-mRFP spot (left bar) and the distances from the center of each ARA6-mRFP spot to the center of the closest ARA6-GFP spot (middle bar) were classified as described previously (Ito et al. 2012). The graph shows that there were no differences between the two measurements (p>0.5 by Student’s t-test). On the other hand, the frequency of colocalization was significantly lower for ARA6-mRFP/GFP-PTS1 (right bar) (p***<0.001 by Tukey’s test). Error bars indicate±standard deviation.