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. 2019 Jul 11;10:1480. doi: 10.3389/fimmu.2019.01480

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Copyright © 2019 Bui, Kolodziej, Lamanna, Elgass, Sehgal, Rudloff, Schwenke, Tsuchimochi, Kroon, Cho, Maksimenko, Cholewa, Berger, Young, Bourke, Pearson, Nold and Nold-Petry.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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α-SMA and ET_A abundance in pulmonary arteries is not altered following hyperoxia. Immunohistochemistry for α-SMA and ET_A was performed in lung sections from the air vehicle and hyperoxia vehicle groups. Each representative image for α-SMA (A) shows an airway on the left and an artery on the right. Scale bars = 100 μm. Representative images for ET_A (C) show an artery only. Scale bar = 50 μm. Staining was analyzed using the Aperio positive pixel count algorithm and expressed as intensity of strongly positive staining divided by area (μm²) for α-SMA (B) and ET_A (D). Data are shown as mean ± SEM, n = 13–20 mice per group.