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. 2019 Jul 9;7:279. doi: 10.3389/fped.2019.00279

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Copyright © 2019 Zhang, Goswami, Ma, Meng, Wang, Zhu, Zhang, Zheng, Dong, Xiao, Zhang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypes, RT-qPCR and apoptosis analyses of ICOS⁺Tregs and ICOS⁻Tregs. (A) Representative images of phenotypic expression of Treg subsets in peripheral blood (upper) and liver tissue (lower) from BA patients. Gates were set based on the ISO. (B,C) The statistical analysis of phenotypic expression of Treg subsets from peripheral blood (upper) and liver tissue (lower). Wilcoxon test was used. (D) RT-qPCR validation of ICOS, CD25, CD39, CD73, TGF-β, and BCL-2 genes were analyzed. Wilcoxon test was used. (E) Apoptosis Assay of Treg subsets from peripheral blood (upper) and liver tissues (lower) of BA patients. Mann-Whitney test was used.